Hotspot

psaI-ycf4

LSC · 60108-60534 · 427 bp

Back to Crateva

Pi 0.00781

MarkerSeek score 62.30

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 427
variable_sites: 5
pi: 0.00781
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GGCAATTGCAATGGCTTCTT	GGATAAAGGCCCAGCAGAAA	45.0	50.0	57.9	57.9	556-564 bp	1.0	79.9
psaI-ycf4_p2	GGCAATTGCAATGGCTTCTT	AAAGGATAAAGGCCCAGCAG	45.0	50.0	57.9	57.9	559-567 bp	1.0	79.8
psaI-ycf4_p3	TAGTCTTTCCGGCAATTGCA	GGATAAAGGCCCAGCAGAAA	45.0	50.0	57.8	57.9	566-574 bp	1.0	79.1
psaI-ycf4_p4	TAGTCTTTCCGGCAATTGCA	AAAGGATAAAGGCCCAGCAG	45.0	50.0	57.8	57.9	569-577 bp	1.0	79.1
psaI-ycf4_p5	AGTAGGCCTAGTCTTTCCGG	GGATAAAGGCCCAGCAGAAA	55.0	50.0	58.2	57.9	574-582 bp	1.0	78.9