Hotspot

ycf3-trnS-UCC

LSC · 44341-45428 · 1088 bp

Back to Crateva

Pi 0.0141

MarkerSeek score 54.90

Primer available yes

Label ycf3-trnS-UCC

Feature Metadata

label_name: ycf3-trnS-UCC
feature_type: igs
region: LSC
length_bp: 1088
variable_sites: 23
pi: 0.0141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-UCC_p1	AATTTCCTTCGGATTGAGCC	TGTTACGCCTTGAACCACTC	45.0	50.0	56.1	58.1	1145-1153 bp	1.0	65.3
ycf3-trnS-UCC_p2	AATTTCCTTCGGATTGAGCC	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.1	58.2	1201-1209 bp	1.0	65.0
ycf3-trnS-UCC_p3	AATTTCCTTCGGATTGAGCC	ACAGTTCCAATGTTACGCCT	45.0	45.0	56.1	57.4	1155-1163 bp	1.0	61.8
ycf3-trnS-UCC_p4	AATTTCCTTCGGATTGAGCC	ATGTTACGCCTTGAACCACT	45.0	45.0	56.1	57.4	1146-1154 bp	1.0	61.8
ycf3-trnS-UCC_p5	AATTTCCTTCGGATTGAGCC	CCAATGTTACGCCTTGAACC	45.0	50.0	56.1	57.4	1149-1157 bp	1.0	61.2