Hotspot

trnD-GAC-trnY-UAC

LSC · 30478-30941 · 464 bp

Back to Crateva

Pi 0.0129

MarkerSeek score 64.40

Primer available yes

Label trnD-GAC-trnY-UAC

Feature Metadata

label_name: trnD-GAC-trnY-UAC
feature_type: igs
region: LSC
length_bp: 464
variable_sites: 9
pi: 0.0129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GAC-trnY-UAC_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	534-540 bp	1.0	75.8
trnD-GAC-trnY-UAC_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	533-539 bp	1.0	75.8
trnD-GAC-trnY-UAC_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	555-561 bp	1.0	74.8
trnD-GAC-trnY-UAC_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	554-560 bp	1.0	74.6
trnD-GAC-trnY-UAC_p5	CGGTGCTCTGACCAATTGAA	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.5	58.4	588-594 bp	1.0	74.2