Hotspot

trnR-AGA-atpA

LSC · 9924-10197 · 274 bp

Back to Crateva

Pi 0.037
MarkerSeek score 66.10
Primer available yes
Label trnR-AGA-atpA

Feature Metadata

label_name
trnR-AGA-atpA
feature_type
igs
region
LSC
length_bp
274
variable_sites
12
pi
0.037
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.92
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-AGA-atpA_p1 AGTGAAAAGCGTCCATTGTCT TCAAGAGCAACTGGAACGTT 42.9 45.0 58.1 57.7 381-398 bp 1.0 74.7
trnR-AGA-atpA_p2 AGTGAAAAGCGTCCATTGTCT TTCAAGAGCAACTGGAACGT 42.9 45.0 58.1 57.7 382-399 bp 1.0 74.6
trnR-AGA-atpA_p3 AGTGAAAAGCGTCCATTGTC TCAAGAGCAACTGGAACGTT 45.0 45.0 56.6 57.7 381-398 bp 1.0 73.0
trnR-AGA-atpA_p4 GTGAAAAGCGTCCATTGTCT TCAAGAGCAACTGGAACGTT 45.0 45.0 56.6 57.7 380-397 bp 1.0 73.0
trnR-AGA-atpA_p5 GTGAAAAGCGTCCATTGTCT TTCAAGAGCAACTGGAACGT 45.0 45.0 56.6 57.7 381-398 bp 1.0 73.0