Hotspot

trnS-AGC-trnG-GGA

LSC · 8258-8864 · 607 bp

Back to Crateva

Pi 0.0121

MarkerSeek score 61.90

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 607
variable_sites: 10
pi: 0.0121
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	646-682 bp	1.0	56.7
trnS-AGC-trnG-GGA_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	645-681 bp	1.0	56.7
trnS-AGC-trnG-GGA_p3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	649-685 bp	1.0	55.6
trnS-AGC-trnG-GGA_p4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	648-684 bp	1.0	55.6
trnS-AGC-trnG-GGA_p5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	647-683 bp	1.0	55.6