Hotspot

trnK-AAA-rps16

LSC · 4290-5255 · 966 bp

Back to Crateva

Pi 0.0186

MarkerSeek score 57.80

Primer available yes

Label trnK-AAA-rps16

Feature Metadata

label_name: trnK-AAA-rps16
feature_type: igs
region: LSC
length_bp: 966
variable_sites: 27
pi: 0.0186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-rps16_p1	CCATTTATTCGCTGGACCCA	AAAAAGGCGCTCAACCTACA	50.0	45.0	57.9	58.0	1325-1340 bp	1.0	82.0
trnK-AAA-rps16_p2	TGGAATCAGGGAAAGAGGGT	AAAAAGGCGCTCAACCTACA	50.0	45.0	57.9	58.0	1356-1371 bp	1.0	82.0
trnK-AAA-rps16_p3	CCATTTATTCGCTGGACCCA	CCCAATGAGCCGTTTATCGA	50.0	50.0	57.9	58.1	1463-1478 bp	1.0	81.7
trnK-AAA-rps16_p4	CCATTTATTCGCTGGACCCA	TCCCAATGAGCCGTTTATCG	50.0	50.0	57.9	58.1	1464-1479 bp	1.0	81.7
trnK-AAA-rps16_p5	TGGAATCAGGGAAAGAGGGT	CCCAATGAGCCGTTTATCGA	50.0	50.0	57.9	58.1	1494-1509 bp	1.0	81.6