Hotspot

rpl32-trnL-UAG

Genome · 117734-119128 · 1395 bp

Back to Crataegus

Pi 0.00767

MarkerSeek score 62.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: Genome
length_bp: 1395
variable_sites: 54
pi: 0.00767
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1443-1586 bp	1.0	85.4
rpl32-trnL-UAG_p2	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1444-1587 bp	1.0	85.4
rpl32-trnL-UAG_p3	TAGGGCAGCATTGAAAGCTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1445-1588 bp	1.0	85.0
rpl32-trnL-UAG_p4	TAGGGCAGCATTGAAAGCTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1446-1589 bp	1.0	85.0
rpl32-trnL-UAG_p5	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.8	57.9	1453-1596 bp	1.0	84.4