Hotspot

ndhF-rpl32

Genome · 116478-117574 · 1097 bp

Back to Crataegus

Pi 0.00842

MarkerSeek score 65.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 1097
variable_sites: 38
pi: 0.00842
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	40.0	45.0	57.0	57.8	1254-1320 bp	1.0	76.7
ndhF-rpl32_p2	TTGTTGCCGTTGGAAAAAGT	AAGCTTTCAATGCTGCCCTA	40.0	45.0	57.0	57.8	1252-1318 bp	1.0	76.4
ndhF-rpl32_p3	TTGTTGCCGTTGGAAAAAGT	GCTGCCCTATATCCCTTCCT	40.0	55.0	57.0	58.3	1241-1307 bp	1.0	75.4
ndhF-rpl32_p4	TTGTTGCCGTTGGAAAAAGT	AGCTTTCAATGCTGCCCTAT	40.0	45.0	57.0	57.6	1251-1317 bp	1.0	74.5
ndhF-rpl32_p5	TTTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	38.1	45.0	57.7	57.8	1255-1321 bp	1.0	74.5