Hotspot

rpl14-rpl16

Genome · 84698-84849 · 152 bp

Back to Crataegus

Pi 0.00744

MarkerSeek score 53.40

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: Genome
length_bp: 152
variable_sites: 10
pi: 0.00744
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CCGCTGTTATCCGCTACATT	TGAAATGAGCGGAGTAGCAG	50.0	50.0	58.1	57.7	288-296 bp	1.0	81.2
rpl14-rpl16_p2	TACACATCAATTCTCGGGCC	TGAAATGAGCGGAGTAGCAG	50.0	50.0	57.7	57.7	308-316 bp	1.0	79.4
rpl14-rpl16_p3	CCGCTGTTATCCGCTACATT	GAAATGAGCGGAGTAGCAGA	50.0	50.0	58.1	57.4	287-295 bp	1.0	78.9
rpl14-rpl16_p4	TACACATCAATTCTCGGGCC	GAAATGAGCGGAGTAGCAGA	50.0	50.0	57.7	57.4	307-315 bp	1.0	77.1
rpl14-rpl16_p5	TCCGCTACATTCAAATGGGT	TGAAATGAGCGGAGTAGCAG	45.0	50.0	57.2	57.7	279-287 bp	1.0	76.0