Hotspot

accD-psaI

Genome · 61971-62762 · 792 bp

Back to Crataegus

Pi 0.0082

MarkerSeek score 48.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: Genome
length_bp: 792
variable_sites: 40
pi: 0.0082
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCATGCAAGAAGGAAGCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1175-1328 bp	1.0	86.5
accD-psaI_p2	GCATGCAAGAAGGAAGCTTG	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.0	58.0	1166-1319 bp	1.0	86.3
accD-psaI_p3	GCATGCAAGAAGGAAGCTTG	GTTTTTCGAGACCCCGCTAT	50.0	50.0	58.0	58.0	1684-1828 bp	1.0	86.2
accD-psaI_p4	GCATGCAAGAAGGAAGCTTG	TCGAGACCCCGCTATAAGTT	50.0	50.0	58.0	57.9	1679-1823 bp	1.0	85.9
accD-psaI_p5	GCATGCAAGAAGGAAGCTTG	TTCGAGACCCCGCTATAAGT	50.0	50.0	58.0	57.9	1680-1824 bp	1.0	85.9