Hotspot

ndhC-trnV-UAC

Genome · 54066-54653 · 588 bp

Back to Crataegus

Pi 0.03
MarkerSeek score 68.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
Genome
length_bp
588
variable_sites
99
pi
0.03
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.94
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCCCATTGGTTCTATTCCCG TCAAGCAGGGCGATAGAAAC 55.0 50.0 58.0 58.0 1014-1115 bp 1.0 91.6
ndhC-trnV-UAC_p2 CCCCATTGGTTCTATTCCCG CAAGCAGGGCGATAGAAACT 55.0 50.0 58.0 58.0 1013-1114 bp 1.0 91.5
ndhC-trnV-UAC_p3 AGAGAAGCGGCCATAAACTG TCAAGCAGGGCGATAGAAAC 50.0 50.0 58.0 58.0 1392-1489 bp 1.0 89.5
ndhC-trnV-UAC_p4 AGAGAAGCGGCCATAAACTG CAAGCAGGGCGATAGAAACT 50.0 50.0 58.0 58.0 1391-1488 bp 1.0 89.5
ndhC-trnV-UAC_p5 TTGTTACTGTGCCTGCTGTT TCAAGCAGGGCGATAGAAAC 45.0 50.0 57.9 58.0 1524-1621 bp 1.0 88.9