Hotspot

trnF-GAA-ndhJ

Genome · 51660-52358 · 699 bp

Back to Crataegus

Pi 0.00425

MarkerSeek score 51.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: Genome
length_bp: 699
variable_sites: 15
pi: 0.00425
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	866-889 bp	1.0	82.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	830-853 bp	1.0	81.8
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	854-877 bp	1.0	81.1
trnF-GAA-ndhJ_p4	GTCGTGAATGGTCGGGATAG	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	879-902 bp	1.0	81.0
trnF-GAA-ndhJ_p5	GAGGATGATGCGTCGTGAAT	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	890-913 bp	1.0	80.9