Hotspot

trnT-UGU-trnL-UAA

Genome · 49314-50576 · 1263 bp

Back to Crataegus

Pi 0.0136

MarkerSeek score 70.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: Genome
length_bp: 1263
variable_sites: 91
pi: 0.0136
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	1169-1378 bp	1.0	88.7
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	1177-1386 bp	1.0	88.1
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	1201-1410 bp	1.0	87.7
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	1209-1418 bp	1.0	87.2
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	1183-1392 bp	1.0	86.7