Hotspot

psbZ-trnG-UCC

Genome · 38763-39228 · 466 bp

Back to Crataegus

Pi 0.0159

MarkerSeek score 65.80

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: Genome
length_bp: 466
variable_sites: 33
pi: 0.0159
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	368-721 bp	1.0	84.7
psbZ-trnG-UCC_p2	GGGTTGGTCGGGTAACAAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	354-707 bp	1.0	84.6
psbZ-trnG-UCC_p3	TTCCGGCACATCATTATGGA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.0	57.8	324-677 bp	1.0	79.1
psbZ-trnG-UCC_p4	AGGGTTGGTCGGGTAACAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	59.2	57.8	355-708 bp	1.0	77.8
psbZ-trnG-UCC_p5	ATTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.2	57.8	369-722 bp	1.0	77.3