Hotspot

psbM-trnD-GUC

Genome · 31447-32684 · 1238 bp

Back to Crataegus

Pi 0.00382

MarkerSeek score 53.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: Genome
length_bp: 1238
variable_sites: 28
pi: 0.00382
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	1385-1417 bp	1.0	75.8
psbM-trnD-GUC_p2	AATCACCTTGGCTGACTGTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.3	58.5	1387-1419 bp	1.0	74.0
psbM-trnD-GUC_p3	ATCACCTTGGCTGACTGTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.3	58.5	1386-1418 bp	1.0	74.0
psbM-trnD-GUC_p4	TCACCTTGGCTGACTGTTTT	GTTCAATTGGTCAGAGCACC	45.0	50.0	57.6	57.0	1386-1418 bp	1.0	71.6
psbM-trnD-GUC_p5	ATCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	42.9	50.0	58.1	58.5	1386-1418 bp	1.0	70.9