Hotspot

trnR-UCU-atpA

Genome · 10102-10782 · 681 bp

Back to Crataegus

Pi 0.015

MarkerSeek score 63.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: Genome
length_bp: 681
variable_sites: 44
pi: 0.015
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAATGAAAAGCGTCCATTG	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	436-850 bp	1.0	73.1
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	435-849 bp	1.0	73.1
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	47.6	50.0	57.3	58.4	375-789 bp	1.0	72.5
trnR-UCU-atpA_p4	TGGATAGGACAGAGGTCTTCT	AGACATTTACCGACGAAGCG	47.6	50.0	56.9	58.4	410-824 bp	1.0	69.4
trnR-UCU-atpA_p5	TGGAATGAAAAGCGTCCATT	AGACATTTACCGACGAAGCG	40.0	50.0	55.9	58.4	436-850 bp	1.0	69.0