Hotspot

rps16-trnQ-UUG

Genome · 6377-7182 · 806 bp

Back to Crataegus

Pi 0.011

MarkerSeek score 56.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 806
variable_sites: 36
pi: 0.011
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	879-909 bp	1.0	85.0
rps16-trnQ-UUG_p2	CGTTGCTTTCTTCCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	860-890 bp	1.0	84.8
rps16-trnQ-UUG_p3	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	889-919 bp	1.0	83.3
rps16-trnQ-UUG_p4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	890-920 bp	1.0	83.2
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	GTGTCGACTAGAAATGGGGC	50.0	55.0	58.2	58.3	941-971 bp	1.0	82.6