Hotspot

psbE-petL

LSC · 60293-61313 · 1021 bp

Back to Crassula

Pi 0.0505

MarkerSeek score 83.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1021
variable_sites: 174
pi: 0.0505
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	CCGAAATAACCAGTTATAGTAGGC	50.0	41.7	57.8	57.0	1057-1077 bp	1.0	51.9
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	CCGAAATAACCAGTTATAGTAGGC	55.0	41.7	58.2	57.0	1052-1072 bp	1.0	51.9
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	CCGAAATAACCAGTTATAGTAGGC	50.0	41.7	58.4	57.0	1050-1070 bp	1.0	51.9
psbE-petL_p4	TTCTCCTGTGCTTCCAGACA	CCGAAATAACCAGTTATAGTAGGC	50.0	41.7	58.6	57.0	1051-1071 bp	1.0	51.9
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	CCGAAATAACCAGTTATAGTAGGCA	50.0	40.0	57.8	58.5	1057-1077 bp	1.0	51.9