Hotspot

petA-psbJ

LSC · 58756-59526 · 771 bp

Back to Crassula

Pi 0.0471

MarkerSeek score 75.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 120
pi: 0.0471
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTTCAATTATCCGAAATGAATTTCT	CTGGTATTGCTGTGATCGGT	28.0	50.0	54.7	57.7	871-1086 bp	1.0	52.0
petA-psbJ_p2	TCAATTATCCGAAATGAATTTCT	CTGGTATTGCTGTGATCGGT	26.1	50.0	52.5	57.7	869-1084 bp	1.0	52.0
petA-psbJ_p3	GTTCAATTATCCGAAATGAATTTC	CTGGTATTGCTGTGATCGGT	29.2	50.0	53.4	57.7	871-1086 bp	1.0	52.0
petA-psbJ_p4	TTCAATTATCCGAAATGAATTTCT	CTGGTATTGCTGTGATCGGT	25.0	50.0	53.3	57.7	870-1085 bp	1.0	52.0
petA-psbJ_p5	GTTCAATTATCCGAAATGAATTTCT	TGGTATTGCTGTGATCGGTT	28.0	45.0	54.7	57.2	870-1085 bp	1.0	52.0