Hotspot

trnF-GAA-ndhJ

LSC · 45272-45942 · 671 bp

Back to Crassula

Pi 0.0659

MarkerSeek score 76.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 141
pi: 0.0659
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	TCCCTCTATCCCCAAATCCC	GTTGGATAGGATGGCCCTTG	55.0	55.0	57.9	58.0	1078-1110 bp	0.75	74.4
trnF-GAA-ndhJ_p2	CCTCTATCCCCAAATCCCCT	GTTGGATAGGATGGCCCTTG	55.0	55.0	57.9	58.0	1076-1108 bp	0.75	74.4
trnF-GAA-ndhJ_p3	TCCCTCTATCCCCAAATCCC	AAAGATGCAGGGTCGTTTGT	55.0	45.0	57.9	58.0	1488-1520 bp	0.75	73.0
trnF-GAA-ndhJ_p4	CGAAAAAGGGGATAGGTGCA	GTTGGATAGGATGGCCCTTG	50.0	55.0	57.9	58.0	1390-1477 bp	0.666667	70.1
trnF-GAA-ndhJ_p5	CGAAAAAGGGGATAGGTGCA	AAAGATGCAGGGTCGTTTGT	50.0	45.0	57.9	58.0	1800-1887 bp	0.666667	69.1