Hotspot

psbZ-trnG-GCC

LSC · 34162-34746 · 585 bp

Back to Crassula

Pi 0.064
MarkerSeek score 81.50
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
585
variable_sites
124
pi
0.064
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CAACCACTCAGCCATCTCTC AGAGAGGCCCCTTTACCATT 55.0 50.0 58.0 58.0 1504-1566 bp 1.0 91.7
psbZ-trnG-GCC_p2 CAACCACTCAGCCATCTCTC AGAGGCCCCTTTACCATTCT 55.0 50.0 58.0 58.0 1502-1564 bp 1.0 91.7
psbZ-trnG-GCC_p3 CAACCACTCAGCCATCTCTC CTTGAGGTCACGGGTTCAAA 55.0 50.0 58.0 58.0 1351-1413 bp 1.0 91.5
psbZ-trnG-GCC_p4 TTTTCTGGCTCGGCTATTCC AGAGAGGCCCCTTTACCATT 50.0 50.0 58.0 58.0 1666-1690 bp 0.833333 76.9
psbZ-trnG-GCC_p5 TTTTCTGGCTCGGCTATTCC AGAGGCCCCTTTACCATTCT 50.0 50.0 58.0 58.0 1664-1688 bp 0.833333 76.9