Hotspot

psbM-trnD-GUC

LSC · 28405-29331 · 927 bp

Back to Crassula

Pi 0.047

MarkerSeek score 81.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 927
variable_sites: 140
pi: 0.047
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGCAGTAGGAACTAAAATGAACA	TCAATTGGTAAGAGCACCGC	37.5	50.0	58.1	58.6	827-1057 bp	1.0	54.6
psbM-trnD-GUC_p2	GGCAGTAGGAACTAAAATGAACAG	TCAATTGGTAAGAGCACCGC	41.7	50.0	57.8	58.6	826-1056 bp	1.0	54.1
psbM-trnD-GUC_p3	GGCAGTAGGAACTAAAATGAACA	TCAATTGGTAAGAGCACCGC	39.1	50.0	56.8	58.6	826-1056 bp	1.0	53.9
psbM-trnD-GUC_p4	AGGCAGTAGGAACTAAAATGAAC	TCAATTGGTAAGAGCACCGC	39.1	50.0	56.5	58.6	827-1057 bp	1.0	52.0
psbM-trnD-GUC_p5	AGTAAAAAGGCAGTAGGAACT	TCAATTGGTAAGAGCACCGC	38.1	50.0	54.5	58.6	834-1064 bp	1.0	52.0