Hotspot

petN-psbM

LSC · 27281-28299 · 1019 bp

Back to Crassula

Pi 0.0401

MarkerSeek score 76.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1019
variable_sites: 134
pi: 0.0401
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	CCTTCGGGTCCAAAGAAACA	45.0	50.0	58.0	58.0	1739-1762 bp	0.833333	77.0
petN-psbM_p2	ATCAAGCGCGAAAGAGATGT	CCTTCGGGTCCAAAGAAACA	45.0	50.0	58.0	58.0	2380-2398 bp	0.333333	55.1
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	TCGTTTCATTTCGGTGGGTT	45.0	45.0	58.0	58.0	1601-1602 bp	0.166667	46.8
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TTCGTTTCATTTCGGTGGGT	45.0	45.0	58.0	58.0	1602-1603 bp	0.166667	46.8
petN-psbM_p5	ATCAAGCGCGAAAGAGATGT	TCGTTTCATTTCGGTGGGTT	45.0	45.0	58.0	58.0	2240-2243 bp	0.166667	46.6