Hotspot

atpH-atpI

LSC · 13568-14010 · 443 bp

Back to Crassula

Pi 0.0657

MarkerSeek score 75.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 95
pi: 0.0657
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCAGCAATAACAGAAGCAGC	GAATACGGTCCTTGGGTTCC	50.0	55.0	58.1	58.0	950-1152 bp	1.0	89.7
atpH-atpI_p2	GCAGCAATAACAGAAGCAGC	TCAACCGCTATCGCTATTCG	50.0	50.0	58.1	58.0	1061-1263 bp	1.0	89.4
atpH-atpI_p3	GGGTTGTCTCGCAATACCTT	GAATACGGTCCTTGGGTTCC	50.0	55.0	57.9	58.0	1041-1243 bp	1.0	89.4
atpH-atpI_p4	AAGAAGGGGCAGGAAAAAGG	GAATACGGTCCTTGGGTTCC	50.0	55.0	58.0	58.0	1321-1331 bp	0.833333	76.0
atpH-atpI_p5	AAGAAGGGGCAGGAAAAAGG	TCAACCGCTATCGCTATTCG	50.0	50.0	58.0	58.0	1432-1442 bp	0.833333	75.6