Hotspot

trnR-UCU-atpA

LSC · 9978-10115 · 138 bp

Back to Crassula

Pi 0.0763
MarkerSeek score 74.00
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
138
variable_sites
34
pi
0.0763
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.93
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 CCCTTCTATGGGGGTCATCT ATCTGTCCAAGCAAGCACAA 55.0 45.0 57.9 58.0 1329-1380 bp 1.0 87.8
trnR-UCU-atpA_p2 CCTCAAGATTCTCGCCACTC ATCTGTCCAAGCAAGCACAA 55.0 45.0 58.1 58.0 1391-1401 bp 0.833333 76.3
trnR-UCU-atpA_p3 CCTCAAGATTCTCGCCACTC ACTGACGAAGCAAAAACCCT 55.0 45.0 58.1 57.9 734-742 bp 0.666667 70.2
trnR-UCU-atpA_p4 CCCTTCTATGGGGGTCATCT ACTGACGAAGCAAAAACCCT 55.0 45.0 57.9 57.9 672-680 bp 0.666667 70.0
trnR-UCU-atpA_p5 CCTCAAGATTCTCGCCACTC CACTGACGAAGCAAAAACCC 55.0 50.0 58.1 57.9 735-743 bp 0.666667 69.6