Hotspot

ycf1

SSC · 123305-128605 · 5301 bp

Back to Crambe

Pi 0.0102
MarkerSeek score 49.70
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5301
variable_sites
54
pi
0.0102
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGAGTGATCTGTTTATTCACA CGGGTCTTGCCAATCAAAAA 33.3 45.0 52.2 57.2 211 bp 1.0 42.7
ycf1_p2 AGAGTGATCTGTTTATTCACA ACGGGTCTTGCCAATCAAAA 33.3 45.0 52.2 58.3 212 bp 1.0 42.6
ycf1_p3 AGAGTGATCTGTTTATTCACAT ACGGGTCTTGCCAATCAAAA 31.8 45.0 52.9 58.3 212 bp 1.0 42.6
ycf1_p4 AGAGTGATCTGTTTATTCACA GACGGGTCTTGCCAATCAAA 33.3 50.0 52.2 58.8 213 bp 1.0 42.6
ycf1_p5 AGAGTGATCTGTTTATTCACATT ACGGGTCTTGCCAATCAAAA 30.4 45.0 53.8 58.3 212 bp 1.0 42.6