Hotspot

rpl16

LSC · 80586-82069 · 1484 bp

Back to Crambe

Pi 0.0162
MarkerSeek score 57.00
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1484
variable_sites
24
pi
0.0162
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCTTTTAATGCAAAGGACGAAGT ACATTTCCATGAAAACTCCTTTGA 34.8 33.3 57.0 57.3 1628-1632 bp 1.0 41.4
rpl16_p2 CTCTTCTTTTAATGCAAAGGACGA ACATTTCCATGAAAACTCCTTTGA 37.5 33.3 57.8 57.3 1632-1636 bp 1.0 41.4
rpl16_p3 TCTCTTCTTTTAATGCAAAGGACG ACATTTCCATGAAAACTCCTTTGA 37.5 33.3 57.8 57.3 1633-1637 bp 1.0 41.4
rpl16_p4 TCTTCTTTTAATGCAAAGGACGA ACATTTCCATGAAAACTCCTTTGA 34.8 33.3 56.7 57.3 1631-1635 bp 1.0 41.4
rpl16_p5 TTCTTTTAATGCAAAGGACGAAGT ACATTTCCATGAAAACTCCTTTGA 33.3 33.3 57.6 57.3 1629-1633 bp 1.0 41.4