Hotspot

petA-psbJ

LSC · 61686-62792 · 1107 bp

Back to Crambe

Pi 0.0235
MarkerSeek score 72.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1107
variable_sites
25
pi
0.0235
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC CAGGATTAGGTTCATCCCTGT 50.0 47.6 58.0 56.8 1217-1249 bp 1.0 66.3
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC TCAGGATTAGGTTCATCCCTGT 50.0 45.5 58.0 58.3 1218-1250 bp 1.0 65.9
petA-psbJ_p3 CATTACGTGTCCAAGGCCTT CAGGATTAGGTTCATCCCTGT 50.0 47.6 58.2 56.8 1214-1246 bp 1.0 65.1
petA-psbJ_p4 CATTACGTGTCCAAGGCCTT TCAGGATTAGGTTCATCCCTGT 50.0 45.5 58.2 58.3 1215-1247 bp 1.0 64.8
petA-psbJ_p5 ATCCATTACGTGTCCAAGGC TCAGGATTAGGTTCATCCCTG 50.0 47.6 58.0 56.5 1218-1250 bp 1.0 64.0