Hotspot

psaI-ycf4

LSC · 58326-58755 · 430 bp

Back to Crambe

Pi 0.0272
MarkerSeek score 75.20
Primer available yes
Label psaI-ycf4

Feature Metadata

label_name
psaI-ycf4
feature_type
igs
region
LSC
length_bp
430
variable_sites
11
pi
0.0272
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-ycf4_p1 GCACTTGCAATGGCTTCTTT TTGTTTTTCGAGACCCCGTT 45.0 45.0 57.8 58.0 527-542 bp 1.0 83.8
psaI-ycf4_p2 AGTAGGCCTAGTCTTTCCGG TTGTTTTTCGAGACCCCGTT 55.0 45.0 58.2 58.0 546-561 bp 1.0 83.1
psaI-ycf4_p3 GCACTTGCAATGGCTTCTTT GGATAAAGGCCCAGCAGAAA 45.0 50.0 57.8 57.9 551-566 bp 1.0 82.7
psaI-ycf4_p4 CCTAGTCTTTCCGGCACTTG TTGTTTTTCGAGACCCCGTT 55.0 45.0 58.3 58.0 540-555 bp 1.0 82.7
psaI-ycf4_p5 GCACTTGCAATGGCTTCTTT TAGGATAAAGGCCCAGCAGA 45.0 50.0 57.8 57.8 553-568 bp 1.0 82.3