Hotspot

trnF-GAA-ndhJ

LSC · 47018-47622 · 605 bp

Back to Crambe

Pi 0.0137
MarkerSeek score 64.00
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
605
variable_sites
8
pi
0.0137
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA GAAAGTTGGATAGGGTGGCC 50.0 55.0 58.1 58.2 692-714 bp 1.0 81.2
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA CTTATGATAGCCATCCGCGG 50.0 55.0 58.1 58.4 733-755 bp 1.0 80.1
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGGTGGC 50.0 50.0 58.1 57.5 693-715 bp 1.0 79.0
trnF-GAA-ndhJ_p4 AATCCTCGTGTCACCAGTTC GAAAGTTGGATAGGGTGGCC 50.0 55.0 57.5 58.2 695-717 bp 1.0 78.0
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA GCGGCTGAAACGGATCTTAA 50.0 50.0 58.1 58.6 717-739 bp 1.0 77.9