Hotspot

trnT-UGU-trnL-UAA

LSC · 45396-46205 · 810 bp

Back to Crambe

Pi 0.00994
MarkerSeek score 65.10
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
810
variable_sites
8
pi
0.00994
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCAATTCC 55.0 55.0 57.9 58.2 869-871 bp 1.0 78.9
trnT-UGU-trnL-UAA_p2 TATGGAAAAGCCGGCTATCG CCGTAGCGTCTACCAATTCC 50.0 55.0 57.8 58.2 920-922 bp 1.0 78.6
trnT-UGU-trnL-UAA_p3 AAACTATGGAAAAGCCGGCT CCGTAGCGTCTACCAATTCC 45.0 55.0 57.8 58.2 924-926 bp 1.0 78.2
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 872-874 bp 1.0 78.2
trnT-UGU-trnL-UAA_p5 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCAATTCC 50.0 55.0 57.8 58.2 901-903 bp 1.0 78.0