Hotspot

psbC-trnS-UGA

LSC · 34277-34455 · 179 bp

Back to Crambe

Pi 0.0391
MarkerSeek score 72.40
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
179
variable_sites
7
pi
0.0391
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 TCGTGGGTCATTTATGGCAC GATGGCTCCGGTCTTGAAAA 50.0 50.0 58.3 58.2 361 bp 1.0 78.1
psbC-trnS-UGA_p2 TCGTGGGTCATTTATGGCAC GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.3 58.2 312 bp 1.0 78.0
psbC-trnS-UGA_p3 CCGCAGCAGGATTTGAAAAA GATGGCTCCGGTCTTGAAAA 45.0 50.0 57.6 58.2 322 bp 1.0 76.8
psbC-trnS-UGA_p4 CCGCAGCAGGATTTGAAAAA GGGTTCGAATCCCTCTCTCT 45.0 55.0 57.6 58.2 273 bp 1.0 76.8
psbC-trnS-UGA_p5 TTCGTGGGTCATTTATGGCA GATGGCTCCGGTCTTGAAAA 45.0 50.0 57.5 58.2 362 bp 1.0 76.1