Hotspot

trnY-GUA-trnE-UUC

LSC · 30160-30218 · 59 bp

Back to Crambe

Pi 0.0339
MarkerSeek score 63.10
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
59
variable_sites
2
pi
0.0339
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CCAGCGTAGACATATTGCCA AAGTATGCCCCCATCGTCTA 50.0 50.0 57.8 57.9 202 bp 1.0 78.1
trnY-GUA-trnE-UUC_p2 ACCAGCGTAGACATATTGCC AAGTATGCCCCCATCGTCTA 50.0 50.0 57.8 57.9 203 bp 1.0 78.1
trnY-GUA-trnE-UUC_p3 AGACAGCGAATTATTGGGCC AAGTATGCCCCCATCGTCTA 50.0 50.0 58.3 57.9 236 bp 1.0 77.3
trnY-GUA-trnE-UUC_p4 CGAATTTACAGTCCGTCCCC AAGTATGCCCCCATCGTCTA 55.0 50.0 58.4 57.9 181 bp 1.0 77.3
trnY-GUA-trnE-UUC_p5 ACGAATTTACAGTCCGTCCC AAGTATGCCCCCATCGTCTA 50.0 50.0 57.6 57.9 182 bp 1.0 77.1