Hotspot

psbA-trnK-UUU

LSC · 1345-1610 · 266 bp

Back to Crambe

Pi 0.0263
MarkerSeek score 64.10
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
266
variable_sites
7
pi
0.0263
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 TACAGAAGCGACCCCATAGG AAAATGCAAGCACGGTTTGG 55.0 45.0 58.6 58.4 429-431 bp 1.0 73.2
psbA-trnK-UUU_p2 ATCCAGTTACAGAAGCGACC AAAATGCAAGCACGGTTTGG 50.0 45.0 57.3 58.4 436-438 bp 1.0 72.5
psbA-trnK-UUU_p3 AGTTACAGAAGCGACCCCAT AAAATGCAAGCACGGTTTGG 50.0 45.0 58.7 58.4 432-434 bp 1.0 72.2
psbA-trnK-UUU_p4 TACAGAAGCGACCCCATAGG ACTCCATCCGACTAGTTCCG 55.0 55.0 58.6 58.6 364 bp 1.0 71.9
psbA-trnK-UUU_p5 TACAGAAGCGACCCCATAGG TGAAAAATGCAAGCACGGTT 55.0 40.0 58.6 57.4 432-434 bp 1.0 71.8