Hotspot

trnH-GUG-psbA

LSC · 97-282 · 186 bp

Back to Crambe

Pi 0.0543
MarkerSeek score 79.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
186
variable_sites
10
pi
0.0543
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CTGCCTTAATCCACTTGGCT CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 259-260 bp 1.0 82.1
trnH-GUG-psbA_p2 CTGCCTTAATCCACTTGGCT TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 262-263 bp 1.0 82.0
trnH-GUG-psbA_p3 ACTGCCTTAATCCACTTGGC CCTCTAGACCTAGCTGCTGT 50.0 55.0 58.2 57.9 260-261 bp 1.0 81.9
trnH-GUG-psbA_p4 ACTGCCTTAATCCACTTGGC TTCCCTCTAGACCTAGCTGC 50.0 55.0 58.2 57.9 263-264 bp 1.0 81.8
trnH-GUG-psbA_p5 AATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.7 57.9 252-253 bp 1.0 80.6