Hotspot

rpoC1

LSC · 65259-68061 · 2803 bp

Back to Cousinia

Pi 0.00093

MarkerSeek score 49.70

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2803
variable_sites: 7
pi: 0.00093
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GATTCGCGTAGAAATTCCGC	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.7	58.0	2956 bp	1.0	77.6
rpoC1_p2	GATTCGCGTAGAAATTCCGC	GCTCGTTCGAGAACTACGAT	50.0	50.0	57.7	57.5	2954 bp	1.0	74.3
rpoC1_p3	GATTCGCGTAGAAATTCCGC	TGCTCGTTCGAGAACTACGA	50.0	50.0	57.7	58.8	2955 bp	1.0	71.2
rpoC1_p4	TGGGTTTGAGTCAGTGATTGG	TTGCTCGTTCGAGAACTACG	47.6	50.0	58.1	58.0	2999 bp	1.0	70.8
rpoC1_p5	GGGTTTGAGTCAGTGATTGGA	TTGCTCGTTCGAGAACTACG	47.6	50.0	57.9	58.0	2998 bp	1.0	70.7