Hotspot

petD

LSC · 6441-7623 · 1183 bp

Back to Cousinia

Pi 0.00151

MarkerSeek score 50.30

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1183
variable_sites: 5
pi: 0.00151
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TGAAGCGACTGTTCCCTAGA	ATTTCCCTTGAACTCCGCAA	50.0	45.0	58.1	57.7	1315-1317 bp	1.0	77.5
petD_p2	TGAAGCGACTGTTCCCTAGA	TTTCCCTTGAACTCCGCAAT	50.0	45.0	58.1	57.7	1314-1316 bp	1.0	77.5
petD_p3	CTTTGAAGCGACTGTTCCCT	ATTTCCCTTGAACTCCGCAA	50.0	45.0	58.1	57.7	1318-1320 bp	1.0	77.2
petD_p4	CTTTGAAGCGACTGTTCCCT	TTTCCCTTGAACTCCGCAAT	50.0	45.0	58.1	57.7	1317-1319 bp	1.0	77.2
petD_p5	ACTTTGAAGCGACTGTTCCC	TTTCCCTTGAACTCCGCAAT	50.0	45.0	58.4	57.7	1318-1320 bp	1.0	74.9