Hotspot

rpl32-trnL-UAG

SSC · 112433-113294 · 862 bp

Back to Cousinia

Pi 0.00793
MarkerSeek score 64.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
862
variable_sites
18
pi
0.00793
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGAAAGGATATTGGGCGG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.6 58.0 926-994 bp 1.0 82.5
rpl32-trnL-UAG_p2 AAGGAAAGGATATTGGGCGG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.6 58.0 927-995 bp 1.0 82.5
rpl32-trnL-UAG_p3 CGGCGTTAAAAGCTTTGTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 909-977 bp 1.0 82.3
rpl32-trnL-UAG_p4 CGGCGTTAAAAGCTTTGTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 910-978 bp 1.0 82.3
rpl32-trnL-UAG_p5 AAGGAAAGGATATTGGGCGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.6 57.9 936-1004 bp 1.0 81.5