Hotspot

petN-trnC-GCA

LSC · 73837-74373 · 537 bp

Back to Cousinia

Pi 0.00572

MarkerSeek score 55.00

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 537
variable_sites: 7
pi: 0.00572
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	TTTCCCCAGTTCAAATCCGG	50.0	50.0	58.2	58.1	577-593 bp	1.0	82.0
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	GGGGGACTGCAAATCCTTTT	50.0	50.0	58.2	58.4	595-611 bp	1.0	79.7
petN-trnC-GCA_p3	GCCCAAGCGAGACTTACTAT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.1	58.1	576-592 bp	1.0	76.0
petN-trnC-GCA_p4	ATTAAAGCAGCCCAAGCGAG	TTTCCCCAGTTCAAATCCGG	50.0	50.0	58.9	58.1	585-601 bp	1.0	75.9
petN-trnC-GCA_p5	AGCCCAAGCGAGACTTACTA	ATTGTGTATCGTTTTGGCGG	50.0	45.0	58.2	57.1	634-650 bp	1.0	75.0