Hotspot

trnT-GGU-trnG-UCC

LSC · 53109-53268 · 160 bp

Back to Cousinia

Pi 0.01

MarkerSeek score 51.90

Primer available yes

Label trnT-GGU-trnG-UCC

Feature Metadata

label_name: trnT-GGU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 160
variable_sites: 5
pi: 0.01
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnG-UCC_p1	ATTTGAACCGAGGGCTTACG	ATCAAACCGAAAGACCCCTT	50.0	45.0	58.3	57.0	295 bp	1.0	71.5
trnT-GGU-trnG-UCC_p2	ATTTGAACCGAGGGCTTACG	AATCAAACCGAAAGACCCCT	50.0	45.0	58.3	57.0	296 bp	1.0	71.4
trnT-GGU-trnG-UCC_p3	GGATTTGAACCGAGGGCTTA	AATCAAACCGAAAGACCCCT	50.0	45.0	57.6	57.0	298 bp	1.0	70.3
trnT-GGU-trnG-UCC_p4	GGATTTGAACCGAGGGCTTA	ATCAAACCGAAAGACCCCTT	50.0	45.0	57.6	57.0	297 bp	1.0	70.3
trnT-GGU-trnG-UCC_p5	ATTTGAACCGAGGGCTTACG	GAATCAAACCGAAAGACCCCT	50.0	47.6	58.3	58.2	297 bp	1.0	69.6