Hotspot

trnS-GGA-pafI

LSC · 39249-40163 · 915 bp

Back to Cousinia

Pi 0.00804

MarkerSeek score 61.90

Primer available yes

Label trnS-GGA-pafI

Feature Metadata

label_name: trnS-GGA-pafI
feature_type: igs
region: LSC
length_bp: 915
variable_sites: 25
pi: 0.00804
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-pafI_p1	ACGGAAAGAGAGGGATTCGA	CATTTATCCGCGATCTAGGCA	50.0	47.6	58.2	58.0	1025-1034 bp	1.0	72.9
trnS-GGA-pafI_p2	TTCCAATGCTACGCCTTCAA	CATTTATCCGCGATCTAGGCA	45.0	47.6	57.8	58.0	975-984 bp	1.0	72.7
trnS-GGA-pafI_p3	GGATTCGAACCCTCGGTAAG	CATTTATCCGCGATCTAGGCA	55.0	47.6	57.8	58.0	1013-1022 bp	1.0	72.3
trnS-GGA-pafI_p4	AGTTCCAATGCTACGCCTTC	CATTTATCCGCGATCTAGGCA	50.0	47.6	58.3	58.0	977-986 bp	1.0	72.1
trnS-GGA-pafI_p5	CCTCGGTAAGCAAAAGCCTA	CATTTATCCGCGATCTAGGCA	50.0	47.6	57.6	58.0	1003-1012 bp	1.0	71.0