Pi
0.00755
MarkerSeek score
49.00
Primer available
yes
Label
trnL-UAA-trnT-UGU
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnL-UAA-trnT-UGU_p1 | ACCAATTTCGCCATATCCCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.0 | 57.9 | 572-576 bp | 1.0 | 80.7 |
| trnL-UAA-trnT-UGU_p2 | TAGCGTCTACCAATTTCGCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.1 | 57.9 | 580-584 bp | 1.0 | 80.2 |
| trnL-UAA-trnT-UGU_p3 | ACCAATTTCGCCATATCCCC | GGAATCGAACCGATGACCAT | 50.0 | 50.0 | 58.0 | 57.8 | 604-608 bp | 1.0 | 79.8 |
| trnL-UAA-trnT-UGU_p4 | TAGCGTCTACCAATTTCGCC | GGAATCGAACCGATGACCAT | 50.0 | 50.0 | 58.1 | 57.8 | 612-616 bp | 1.0 | 79.2 |
| trnL-UAA-trnT-UGU_p5 | AGTCCGTAGCGTCTACCAAT | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.2 | 57.9 | 586-590 bp | 1.0 | 78.8 |