Hotspot

trnV-UAC-ndhC

LSC · 32753-33920 · 1168 bp

Back to Cousinia

Pi 0.00431

MarkerSeek score 49.20

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: LSC
length_bp: 1168
variable_sites: 13
pi: 0.00431
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	CCGAGAAAGTCTACGGTTCG	GAGCTAAAACTCCCGAAATGA	55.0	42.9	58.1	56.0	1288-1305 bp	1.0	56.7
trnV-UAC-ndhC_p2	CCGAGAAAGTCTACGGTTCG	GAGCTAAAACTCCCGAAATGAA	55.0	40.9	58.1	56.7	1288-1305 bp	1.0	54.6
trnV-UAC-ndhC_p3	CCGAGAAAGTCTACGGTTCG	AGCTAAAACTCCCGAAATGA	55.0	40.0	58.1	54.7	1287-1304 bp	1.0	54.2
trnV-UAC-ndhC_p4	CCGAGAAAGTCTACGGTTCG	AGCTAAAACTCCCGAAATGAA	55.0	38.1	58.1	55.5	1287-1304 bp	1.0	52.9
trnV-UAC-ndhC_p5	CCGAGAAAGTCTACGGTTCG	GAGCTAAAACTCCCGAAATG	55.0	45.0	58.1	54.4	1288-1305 bp	1.0	52.1