Hotspot

psbJ-petA

LSC · 20226-21046 · 821 bp

Back to Cousinia

Pi 0.00392

MarkerSeek score 62.50

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 8
pi: 0.00392
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	ATTCCGGATTGGGTTCATCC	ACGTGTACAAGGCCTTTTGT	50.0	45.0	57.6	58.0	927-964 bp	1.0	81.4
psbJ-petA_p2	ATTCCGGATTGGGTTCATCC	GGCCTTTTGTTCTTCTTCGC	50.0	50.0	57.6	57.9	917-954 bp	1.0	81.4
psbJ-petA_p3	TGTGATCGGTTTAGTAGGCG	ACGTGTACAAGGCCTTTTGT	50.0	45.0	57.4	58.0	965-1002 bp	1.0	79.7
psbJ-petA_p4	GTGATCGGTTTAGTAGGCGT	ACGTGTACAAGGCCTTTTGT	50.0	45.0	57.4	58.0	964-1001 bp	1.0	79.7
psbJ-petA_p5	TGTGATCGGTTTAGTAGGCG	GGCCTTTTGTTCTTCTTCGC	50.0	50.0	57.4	57.9	955-992 bp	1.0	79.7