Hotspot

petL-psbE

LSC · 18153-19434 · 1282 bp

Back to Cousinia

Pi 0.000919

MarkerSeek score 50.60

Primer available yes

Label petL-psbE

Feature Metadata

label_name: petL-psbE
feature_type: igs
region: LSC
length_bp: 1282
variable_sites: 4
pi: 0.000919
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-psbE_p1	GTCGTATCTTGCTCAGACCA	CAACCCGCAATGAATAGGGA	50.0	50.0	57.1	57.9	1474-1479 bp	1.0	72.5
petL-psbE_p2	GTCGTATCTTGCTCAGACCA	AGAACGTTCTCCTGTGCTTC	50.0	50.0	57.1	57.8	1397-1402 bp	1.0	71.7
petL-psbE_p3	GTCGTATCTTGCTCAGACCA	ACGAATAACCAACCCGCAAT	50.0	45.0	57.1	58.2	1483-1488 bp	1.0	71.5
petL-psbE_p4	GTCGTATCTTGCTCAGACCA	TGCTGACGAATAACCAACCC	50.0	50.0	57.1	58.2	1488-1493 bp	1.0	71.4
petL-psbE_p5	GTCGTATCTTGCTCAGACCA	CGAATAACCAACCCGCAATG	50.0	50.0	57.1	57.8	1482-1487 bp	1.0	71.4