Hotspot

trnD-GUC

LSC · 39754-39827 · 74 bp

Back to Coursetia

Pi 0.479

MarkerSeek score 76.40

Primer available yes

Label trnD-GUC

Feature Metadata

label_name: trnD-GUC
feature_type: tRNA
region: LSC
length_bp: 74
variable_sites: 34
pi: 0.479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC_p1	TCTTCGTCGGAACCTTTACC	AAGTATGCCCCCATCGTCTA	50.0	50.0	57.3	57.9	898 bp	0.5	53.4
trnD-GUC_p2	TCTTCGTCGGAACCTTTACC	CGGCCCAAAAATTTGCTGAT	50.0	45.0	57.3	57.9	682 bp	0.5	53.4
trnD-GUC_p3	CTTCGTCGGAACCTTTACCT	AAGTATGCCCCCATCGTCTA	50.0	50.0	57.3	57.9	897 bp	0.5	53.3
trnD-GUC_p4	CTTCGTCGGAACCTTTACCT	CGGCCCAAAAATTTGCTGAT	50.0	45.0	57.3	57.9	681 bp	0.5	53.3
trnD-GUC_p5	TCTTCGTCGGAACCTTTACC	TTGTTCTCCCAACATGACGG	50.0	50.0	57.3	58.1	615 bp	0.5	53.3