Hotspot

ndhH

SSC · 118336-119517 · 1182 bp

Back to Coursetia

Pi 0.47

MarkerSeek score 78.90

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 433
pi: 0.47
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AAAAAGGAGGGGGAGAGGAA	CCGTCTGCTAGAGCTTGAAG	50.0	55.0	57.9	58.1	1836 bp	0.5	58.2
ndhH_p2	AAAAGGAGGGGGAGAGGAAA	CCGTCTGCTAGAGCTTGAAG	50.0	55.0	57.9	58.1	1835 bp	0.5	58.2
ndhH_p3	AAAAAGGAGGGGGAGAGGAA	TTTGTTCCGTCTGCTAGAGC	50.0	50.0	57.9	57.9	1842 bp	0.5	58.2
ndhH_p4	AAAAGGAGGGGGAGAGGAAA	TTTGTTCCGTCTGCTAGAGC	50.0	50.0	57.9	57.9	1841 bp	0.5	58.2
ndhH_p5	AAAAGGAGGGGGAGAGGAAA	TCAGGTCCAATACGCTGTTG	50.0	50.0	57.9	57.9	1793 bp	0.5	58.1