Hotspot

ndhG

SSC · 122869-123399 · 531 bp

Back to Coursetia

Pi 0.447

MarkerSeek score 79.10

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 199
pi: 0.447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AATCAAATTGCTTTGGGCCG	CCAATCCGATAGCTGCTTCA	45.0	50.0	57.9	57.8	1532 bp	0.5	57.3
ndhG_p2	AATCAAATTGCTTTGGGCCG	GTTGTGTAGCCTGCATGCTA	45.0	50.0	57.9	58.3	1688 bp	0.5	57.1
ndhG_p3	AATCAAATTGCTTTGGGCCG	ATAGCCAATCCGATAGCTGC	45.0	50.0	57.9	57.6	1536 bp	0.5	56.1
ndhG_p4	AATCAAATTGCTTTGGGCCG	TGTTCGAGCATCATTGACCA	45.0	45.0	57.9	57.5	1311 bp	0.5	55.5
ndhG_p5	AATCAAATTGCTTTGGGCCG	TTGTGTAGCCTGCATGCTAA	45.0	45.0	57.9	57.5	1687 bp	0.5	55.3