Hotspot

ndhD

SSC · 124585-126081 · 1497 bp

Back to Coursetia

Pi 0.465

MarkerSeek score 80.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1497
variable_sites: 574
pi: 0.465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGATACCTTGGGACGGATGT	TCAGGAGCAGTATGGGCTAA	50.0	50.0	58.1	57.8	2268 bp	0.5	57.6
ndhD_p2	TGATACCTTGGGACGGATGT	TTCAGGAGCAGTATGGGCTA	50.0	50.0	58.1	57.8	2269 bp	0.5	57.6
ndhD_p3	TGATACCTTGGGACGGATGT	GAGGCGTGGGGATCATATTG	50.0	55.0	58.1	58.2	2247 bp	0.5	57.5
ndhD_p4	AAGAGGTGTGAATCCGCTTG	TCAGGAGCAGTATGGGCTAA	50.0	50.0	58.2	57.8	2197 bp	0.5	57.1
ndhD_p5	AAGAGGTGTGAATCCGCTTG	TTCAGGAGCAGTATGGGCTA	50.0	50.0	58.2	57.8	2198 bp	0.5	57.1