Hotspot

trnL-UAG-rpl32

SSC · 127495-128086 · 592 bp

Back to Coursetia

Pi 0.427

MarkerSeek score 80.30

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 592
variable_sites: 212
pi: 0.427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	GAAGTAGCAAAGCCTTGGGT	GCAATCGATTCTTACGGGGA	50.0	50.0	58.1	57.8	1182 bp	0.5	57.4
trnL-UAG-rpl32_p2	ATGGGATTGTAGAGCGGGTA	GCAATCGATTCTTACGGGGA	50.0	50.0	57.9	57.8	1240 bp	0.5	57.3
trnL-UAG-rpl32_p3	GATAAGATGTCATGCCGCCA	GCAATCGATTCTTACGGGGA	50.0	50.0	58.1	57.8	736 bp	0.5	57.2
trnL-UAG-rpl32_p4	TCCTAAGAGCAGCGTGTCTA	GCAATCGATTCTTACGGGGA	50.0	50.0	57.9	57.8	683 bp	0.5	57.1
trnL-UAG-rpl32_p5	AGATAAGATGTCATGCCGCC	GCAATCGATTCTTACGGGGA	50.0	50.0	57.8	57.8	737 bp	0.5	56.7